FORMATION OF 1-BETA-D-ARABINOFURANOSYLCYTOSINE DIPHOSPHATE CHOLINE IN NEOPLASTIC AND NORMAL CELLS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (6) , 1730-1733
Abstract
1-.beta.-D-Arabinofuranosylcytosine diphosphate choline was formed from 1-.beta.-D-arabinofuranosylcytosine (ara-C) during incubation in vitro of peripheral myeloblasts from patients with acute myelogenous leukemia and cultured cells (nonleukemic human lymphocytes, mouse lymphoma L5178Y and HeLa cervical cancer); as well, 1-.beta.-D-arabinofuranosylcytosine diphosphate choline was formed in vivo in mouse leukemia L1210 cells and mouse liver. 3-Deazauridine enhanced the anabolism of ara-C in nonleukemic lymphocytes in vitro and leukemia L1210 cells in vivo but did not influence ara-C anabolism in the other cell types. In acute myelogenous leukemia myeloblasts incubated in vitro with ara-C, concentrations of 1-.beta.-D-arabinofuranosylcytosine 5''-triphosphate were maximal after 8 h of incubation, and formation of the latter preceded that of 1-.beta.-D-arabinofuranosylcytosine diphosphate choline.