A Simple Stained Protein Assay (SPA) for Rapid Total Protein Determination Applied to Spinal Fluids

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Abstract
A rapid, simple technique measured small amounts of total protein applied on an agarose gel slab. Alternating current through the gel may reduce diffusion of the protein, which was chemically fixed. The intensity of the Coomassie-Blue-stained spots was a function of the amount of protein determined by comparison with simultaneously run standards. Densitometric and visual estimates of protein in [human] spinal fluids by the SPA were significantly correlated with Lowry values (n = 28; P < 0.001, Spearman test of rank correlation). As little as 0.2 .mu.g protein was measured; this figure should be compared with 10 .mu.g for the Lowry method. The reproducibility of the method was .+-. 5%.

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