LEUKOCYTE-AGGREGATION ASSAY FOR TRANSMISSIBLE GASTROENTERITIS OF SWINE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 37  (12) , 1405-1408
Abstract
An in vitro leukocyte-aggregation assay was developed to detect the exposure of swine to transmissible gastroenteritis virus. Leukocytes in heparinized blood samples aggregated when mixed with test antigen prepared from transmissible gastroenteritis-infected swine testicle cell cultures. Of 23 swine exposed .gtoreq. 3 days 22 were positive or suspects in the assay; 6 nonexposed swine were negative. Aggregation was shown as early as 3 days postexposure in 1 sow and persisted for as long as 14 mo. in another. Persistence of the assay was proved by repeated evaluations on 2 experimentally exposed swine.