Field Evaluation of the MUG Assay for Enumerating Escherichia coli in Seawater and Oysters from Southeastern United States

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Abstract
Oysters and seawater collected from the southeastern United States were examined for fecal coliforms and Escherichia coli, using the current procedure of the American Public Health Association (APHA) and the fluorogenic 4-methylumbelliferyl-β-D-glucuronide (MUG) modified APHA procedure. After the presence of E. coli in both methods was confirmed by conventional IMViC procedures, there was no significant difference between method means at the α = 0.05 level. In oysters, low confirmation rates of 67 and 77% were observed by the APHA and the MUG methods, respectively. Seawater had the greatest confirmation rates (95%) by the MUG method. The MUG method may be a suitable alternative to the current APHA method for the microbiological evaluation of oysters and seawater.

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