Contribution of amino acids in protective solutions to postischemic functional recovery of canine kidneys

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Abstract
Amino acids are known to increase glomerular filtration rate (GFR). There is also an early resumption of filtration following 2-h renal ischemic stress under protection by histidine-buffered histidine-tryptophanketoglutarate solution (HTK), possibly due in part to an amino acid effect. Hence, we have examined the possibility of further enhancing the postischemic GFR by adding 32 (ASP I; 4 mM Mg2+) or 36 (ASP II; 6 mM Mg2+) mM l-aspartate (asp) or 32 mM dl-aspartate (ASP III) to the HTK solution in place of chloride. After infusion of 500 ml 5% glucose, canine kidneys were protected by an 8-min perfusion with HTK (n = 5), ASP I (n = 4), ASP II (n = 5) or ASP III-solution (n = 3). The subsequent ischemia lasted for 2 h at 27–31°C. During reperfusion, both GFR and filtration fraction (FF) were higher in kidneys protected byl-aspartate-containing solutions. ASP III showed no improvement against HTK. An additional preischemic intraaortal application of HTK or ASP I solution just above the exit of the renal arteries prior to the intrinsic protective perfusion further raised the postischemic GFR. The present results suggest thatl-aspartate but also histidine may have favorable amino acid effects in renal protective solutions in addition to known positive effects of histidine.

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