A Nanosecond Temperature-Jump Apparatus

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Abstract
A simple apparatus that extends the applicability of the temperature‐jump method for the investigation of fast chemical kinetics in solution from the microsecond to the nanosecond time range is described. A sample solution can be resistively heated a maximum of 10°C within 50 nsec using the discharge of a coaxial cable capacitor. Relaxation of the reaction to a new equilibrium is followed spectrophotometrically. The design of the sample microcell (minimum volume 40 μl) is of particular interest for biochemists working with relaxation kinetics, because the volume of solution required is an order of magnitude less than for previous Joule heated temperature‐jump cells.