IMMUNOASSAY OF BOVINE AND HUMAN PARATHYROID HORMONE

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Abstract
The principle of competitive inhibition of binding of I131-labeled parathyroid hormone to specific hormone antibodies is the basis for an assay of bovine and human parathyroid hormones in the [mu][mu]g-m[mu]g range. Immunoassay of crude preparations of bovine parathyroid hormone using a pure preparation as standard gave results in good agreement with those obtained by bioassay. Human parathyroid hormone extracted from a parathyroid adenoma reacted sufficiently well with guinea pig antibodies to bovine parathyroid hormone to be detectable at concentrations of 1.5-6 mU/ml (250-1,000 [mu][mu]g/ml. Endogenous parathyroid hormone was detected in plasma from 2 hyperparathyroid subjects and in plasma from some normal subjects but not in plasma from 9 patients with hypoparathyroidism.