CHOLESTEROL BIOSYNTHESIS IN AN ADRENAL TUMOR1

Abstract
A human adrenal tumor was shown to utilize Na acetate-1- C14 as substrate for the synthesis of cholesterol in isolated organ perfusion. The presence of unidentified materials, co-precipitated with cholesterol by digitonin and removed from cholesterol only with difficulty, was demonstrated in the tumor sterol fraction. Careful assay of each chromatographic fraction for radioactivity or purification via the dibromide was found to be necessary for a radiochemically pure sample.

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