The glycoprotein 71 of ecotropic Friend murine leukemia virus

Abstract
The glycoprotein from Friend murine leukemia virus was digested with protease from Staphylococcus aureus V8. A glycopeptide comprising the N‐terminal glycosylation site (Asn‐12) was isolated from the mixture of fragments and analyzed by amino acid sequencing and methylation‐capillary gas chromatography—mass spectrometry before and after treatment with sialidase from Vibrio cholerae. Asn‐12 was thus found to be substituted by a family of partially sialylated, fucosylated, and intersected glycoprotein N‐glycans of the hybrid type.

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