Binding of pepsin-treated human immunoglobulin IgG-F(ab')2 to the bacterial cell surface. II: A binding through the antigen-antibody reaction.
- 1 January 1985
- journal article
- research article
- Published by Japan Society of Histochemistry & Cytochemistry in ACTA HISTOCHEMICA ET CYTOCHEMICA
- Vol. 18 (1) , 13-19
- https://doi.org/10.1267/ahc.18.13
Abstract
IgG-F(ab'')2 from normal human serum was incubated and bound to paraformaldehyde fixed bacteria of Klebsiella pneumoniae and Escherichia coli. The IgG-F(ab'')2 that bound to the bacteria was each solubilized from the bacteria by glycine-HCl buffer, pH 2.5, and was again reacted with the paraformaldehyde fixed bacteria. The IgG-F(ab'')2 solubilized from E. coli and K. pneumoniae each showed binding to respective bacteria but not to unrelated bacteria, these findings indicating that the binding of the IgG-F(ab'')2 with bacteria is, if not all, through specific antigen-antibody reaction.This publication has 0 references indexed in Scilit: