Translation of the Satellite RNA of Tomato Black Ring Virus in vitro and in Tobacco Protoplasts

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Abstract
Summary Translation of the satellite RNA (RNA-3) of tomato black ring virus (TBRV) in wheat germ extracts or reticulocyte lysates resulted in the synthesis of a polypeptide of mol. wt. about 48000, both in the presence and in the absence of RNA-1 and RNA-2. The RNA-3 specific polypeptide of TBRV strain G was slightly larger than that of strain S. A polypeptide of the same electrophoretic mobility as the in vitro translation product of RNA-3 was found in extracts of protoplasts infected with an isolate of TBRV-S possessing RNA-3 but not in extracts of protoplasts infected with an isolate lacking RNA-3. The lack of phenotypic effect of RNA-3 raises the question of the function of this protein in the infected plant.