Fascin, an echinoid actin-bundling protein, is a homolog of the Drosophila singed gene product.
Open Access
- 1 October 1993
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (19) , 9115-9119
- https://doi.org/10.1073/pnas.90.19.9115
Abstract
A cDNA for fascin, an actin-bundling protein in echinoderms, has been cloned, sequenced, and expressed. The predicted mass of the protein is approximately 55 kDa, similar to that observed for fascin purified from sea urchin eggs. Bacterially expressed fascin reacts with antibodies prepared against sea urchin egg fascin. Fascin has a strong sequence similarity to the singed gene (sn) product in Drosophila and has similarities with a 55-kDa human actin-bundling protein. No extensive similarities were found with other known actin-binding/bundling proteins, indicating that this is a separate gene family.Keywords
This publication has 26 references indexed in Scilit:
- Automatic generation of primary sequence patterns from sets of related protein sequences.Proceedings of the National Academy of Sciences, 1990
- Hisactophilin, a histidine-rich actin-binding protein from Dictyostelium discoideumJournal of Biological Chemistry, 1989
- Sequence of human villin: a large duplicated domain homologous with other actin-severing proteins and a unique small carboxy-terminal domain related to villin specificity.The Journal of cell biology, 1988
- Villin sequence and peptide map identify six homologous domains.Proceedings of the National Academy of Sciences, 1988
- Formation of filopodia in coelomocytes: Localization of Fascin, a 58,000 Dalton actin cross-linking proteinCell, 1979
- Separation and interaction of the major components of sea urchin actin gelJournal of Molecular Biology, 1978
- Polarized bundles of actin filaments within microvilli of fertilized sea urchin eggs.The Journal of cell biology, 1977
- Structure of actin-containing filaments from two types of non-muscle cellsJournal of Molecular Biology, 1977
- Preparation and purification of polymerized actin from sea urchin egg extracts.The Journal of cell biology, 1975
- The fine structure of developing bristles in wild type and mutant Drosophila melanogasterJournal of Morphology, 1967