SEROLOGY OF NEISSERIA GONORRHOEAE. DEMONSTRATION BY CO‐AGGLUTINATION AND IMMUNOELECTROPHORESIS OF ANTIGENIC DIFFERENCES ASSOCIATED WITH COLOUR/OPACITY COLONIAL VARIANTS

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Abstract
Serological classification of N. gonorrhoeae by co-agglutination (COA) into previously described antigen classes W and J was confirmed. Immunization of rabbits with classified organisms gave antibodies which produced the expected results when used in the preparation of COA reagents. Color/opacity colonial variants of isogenic strains, characterized by stereomicroscopy as opaque and transparent, respectively, influenced immunization, absorption, coagglutination and precipitation in immunoelectrophoresis (IE). COA and IE showed that organisms of opaque colonies often contained an extra antigenic factor(s) which resisted heating at 100.degree. C but was sensitive to treatment with pronase. They were extracted by heating the organisms in saline or LiCl solution. Serological classification with COA of clinical isolates was reproducible with reagents for antigen classes W and J. Colony morphology dependent reactions, mostly due to organisms of opaque colonies, occurred in 7% with reagents for antigen class W and in 20% for antigen class J.