Dielectric spectroscopy of mammalian cells
- 1 January 1993
- journal article
- Published by Springer Nature in Cytotechnology
- Vol. 13 (3) , 185-193
- https://doi.org/10.1007/bf00749814
Abstract
The capacitance of suspensions of CHO and HeLa cells (0.5–3×106 cells/ml) has been measured between 0.2 and 10 MHz. As frequencies decrease, there is a continuous increase in capacitance of both the cell suspension and the spent growth medium free of cells, a phenomenon which is partially attributed to an increased polarisation of the electrodes. At a given frequency, subtraction of the capacitance of the spent medium from that of the cell suspension allows one to determine the capacitance of the cells only. The intensity of this signal varies linearly with the biomass and cell size. At low frequencies such as those used in this study (0.25 MHz), where sensitivity is the highest, concentrations as low as 0.5×106 cells/ml can be accurately measured. Suggestions are made how to make these measures on-line, non-invasive and in real time.Keywords
This publication has 13 references indexed in Scilit:
- On-line biomass monitoring by capacitance measurementJournal of Biotechnology, 1992
- The dielectric permittivity at radio frequencies and the bruggeman probe: novel techniques for the on-line determination of biomass concentrations in plant cell culturesJournal of Biotechnology, 1991
- Substitution and spreadsheet methods for analysing dielectric spectra of biological systemsEuropean Biophysics Journal, 1990
- Real-time monitoring of cellular biomass: Methods and applicationsTrAC Trends in Analytical Chemistry, 1990
- On the audio- and radio-frequency dielectric behaviour of anchorage-independent, mouse L929-derived LS fibroblastsBioelectrochemistry and Bioenergetics, 1988
- Dielectric permittivity of microbial suspensions at radio frequencies: a novel method for the real-time estimation of microbial biomassEnzyme and Microbial Technology, 1987
- Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. I. Dielectric behavior of cell suspensions in 0.01–500 MHz and its simulation with a single-shell modelBiochimica et Biophysica Acta (BBA) - Biomembranes, 1987
- Growth of animal cells on microbeads. II. Estimation of biomass in situ and observation of cytopathic effects after infection of McCoy cells with Chlamydia trachomatisBiology of the Cell, 1987
- Passive electrical properties of the membrane and cytoplasm of cultured rat basophil leukemia cells. II. Effects of osmotic perturbationBiochimica et Biophysica Acta (BBA) - Biomembranes, 1986
- Study of RNA extracted from HeLa cell polysomes. Isopycnic centrifugation of cytoplasmic particles extracted from normal, poliovirus-infected HeLa cells and mouse A9 cellsArchives of Biochemistry and Biophysics, 1972