DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12.
- 1 July 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (13) , 4134-4138
- https://doi.org/10.1073/pnas.81.13.4134
Abstract
The carAB operon of Escherichia coli K-12, which encodes the two subunits of carbamoyl-phosphate synthetase (glutamine hydrolyzing) [carbon-dioxide: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); EC 6.3.5.5], is cumulatively repressed by arginine and the pyrimidines. We describe the structure of the control region of carAB and the sequence of the carA gene. Nuclease S1 mapping experiments show that two adjacent tandem promoters within the carAB control region serve as initiation sites. The upstream promoter P1 is controlled by pyrimidines; the downstream promoter P2 is regulated by arginine. Attenuation control does not appear to be involved in the expression of carAB. A possible mechanism by which control at these promoters concurs to produce a cumulative pattern of repression is discussed. The translational start of carA is atypical; it consists of a UUG or AUU codon.This publication has 52 references indexed in Scilit:
- The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.Proceedings of the National Academy of Sciences, 1983
- Molecular basis for modulated regulation of gene expression in the arginine regulon ofEscherichia coliK-12Nucleic Acids Research, 1983
- E. coli RNA polymerase interacts homologously with two different promotersCell, 1980
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979
- Unusual location and function of the operator in the Escherichia coli galactose operonNature, 1979
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Identification of the UUG codon as a translational initiation codon in vivoJournal of Molecular Biology, 1975
- Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coliMolecular Genetics and Genomics, 1972
- Control of the biosynthesis of carbamoyl phosphate in Escherichia coliJournal of Molecular Biology, 1965
- Regulation and mutation affecting a glutamine dependent formation of carbamyl phosphate in EscherichiacoliBiochemical and Biophysical Research Communications, 1964