Preliminary experiments on the estimation of traces of heteroauxin in soils

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Abstract
A method is described by which small quantities of indolyl acetic acid can be detected and measured quantitatively, depending on the effect of this substance on osmotic pressure of root hair cells of wheat seedlings. Full details are given of the procedure adopted and the precautions which it was found necessary to take, and suggestions are put forward as to how the method might be rendered more accurate if necessary. Possible sources of e"rror are also discussed.An experiment is described in which this method is used to determine what effect is produced by different manurial treatments on the content of indolyl acetic acid in the soil. It was found that farmyard manure tended to increase the quantity present.

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