Antigene und bösartige Geschwülste. V

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Abstract
The effect of insulin, which is not an antigen, and of gelatin converted into an antigen by the introduction of a prosthetic group, on the growth of tumors in mice is studied. The blood sugar reducing action of insulin is removed by the reduction of the S-S bonds with cysteine. To prepare the gelatin antigen, 1-tyrosine ethyl ester, whose amino group is blocked with the carbobenzoxy radical, is converted into o-tetracetyl-B-glucosido-N-carbobenzoyl-1-tyrosine ethyl ester by treatment with acetobromoglucose. This ester is treated with hydrazine hydrate and the corresponding acid hydrazide obtained. The acid hydrazide is converted into the azide in the presence of nitrous acid and the azide coupled with the gelatin. Tumor extract is prepared by hashing sarcomas obtained from mice and shaking the product with an equal vol. of H2O for 24 hrs. and evaporating the extract to dryness in vacuo. The dry residue is dissolved in H2O or 10% glycerin soln. for injection. All the mice used are given a 1 or 2 mg. depot of benzpyrene. More than 70% of the mice treated with benzpyrene alone, or in combination with gelatin, and 50% of the mice injected with 15[gamma] of the gelatin antigen once or twice weekly develop sarcoma. 63% of the mice injected in a similar manner with inactivated insulin, and 40% injected with the tumor extract develop sarcoma. Protein materials should contain carbohydrate residues in order to have antigen properties, while the presence of tyrosine groups does not insure antigen activity. Antigens and the sarcoma extract decrease the development of sarcoma in mice.

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