Immunological identification of a high molecular weight protein as a candidate for the product of the Duchenne muscular dystrophy gene.
Open Access
- 1 June 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (12) , 4491-4495
- https://doi.org/10.1073/pnas.85.12.4491
Abstract
An oligopeptide was synthesized based on translation of the nucleotide sequence of the putative exon region of clone pERT87-25 from the gene for Duchenne muscular dystrophy. Immunization of rabbits with this oligopeptide induced the formation of antibodies directed against a protein present in human, rat, and rabbit skeletal muscle. This protein, which is missing in the skeletal muscle of two patients with Duchenne muscular dystrophy, has a molecular mass of .apprxeq. 320-420 kDa and is clearly different from the putative Duchenne muscular dystrophy-related protein nebulin. The data suggest that this 320- to 420-kDa protein is produced by the Duchenne muscular dystrophy gene.Keywords
This publication has 13 references indexed in Scilit:
- Dystrophin: The protein product of the duchenne muscular dystrophy locusCell, 1987
- Subcellular fractionation of dystrophin to the triads of skeletal muscleNature, 1987
- Complete cloning of the duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individualsCell, 1987
- Mapping the disease phenotypeNature, 1987
- Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy geneNature, 1986
- A physiological role for titin and nebulin in skeletal muscleNature, 1986
- Naturally occurring autoantibodies to skeletal proteins from human red blood cells.The Journal of Immunology, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagentBiochemical Journal, 1978
- Antibodies to tubulin in normal nonimmunized animals.Proceedings of the National Academy of Sciences, 1977