A Method for Increasing the Concentration of a Specific Internal Metabolite in Steady‐State Systems
- 1 December 1994
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 226 (2) , 649-656
- https://doi.org/10.1111/j.1432-1033.1994.tb20092.x
Abstract
An approach is described by which it is possible to increase the concentration of any internal metabolite without affecting the concentrations of other metabolites and fluxes in the organism. This approach requires the manipulation of only a limited number of enzyme activities. The method shows which enzymes to manipulate and the extent of the manipulation required to achieve a given increase in a chosen metabolite. A case study involving tryptophan overproduction in Saccharomyces cerevisae is given as a practical example of how this method could be used.Keywords
This publication has 29 references indexed in Scilit:
- A universal method for achieving increases in metabolite productionEuropean Journal of Biochemistry, 1993
- Hyperproduction of Tryptophan in Corynebacterium glutamicum by Pathway EngineeringNature Biotechnology, 1993
- Responses of metabolic systems to large changes in enzyme activities and effectorsEuropean Journal of Biochemistry, 1993
- Enzyme‐enzyme interactions and control analysisEuropean Journal of Biochemistry, 1990
- Enzyme‐enzyme interactions and control analysisEuropean Journal of Biochemistry, 1990
- Anti-sense RNA as a tool to study plant gene expressionNature, 1988
- Amino acid transport in eucaryotic microorganismsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1986
- Constitutive and Conditional Suppression of Exogenous and Endogenous Genes by Anti-Sense RNAScience, 1985
- Biochemical systems analysisJournal of Theoretical Biology, 1969
- Biochemical systems analysisJournal of Theoretical Biology, 1969