Fast Electron Transfer from Cytochromec6and Plastocyanin to Photosystem I ofChlamydomonasreinhardtiiRequires PsaF
- 1 May 1997
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 36 (21) , 6343-6349
- https://doi.org/10.1021/bi970082c
Abstract
To study the function of the PsaF subunit of photosystem I (PSI), the interactions between plastocyanin or cytochrome c6 and PSI isolated from wild-type and a PsaF-deficient mutant of Chlamydomonas reinhardtii have been examined using cross-linking techniques and flash absorption spectroscopy. We show that efficient electron transfer from both plastocyanin and cytochrome c6 to PSI depends on PsaF. A remarkable feature of the PSI complex of C. reinhardtii is that both plastocyanin and cytochrome c6 reduce P700+ with first-order kinetics and a half-time of 3 μs, which is unique among photosynthetic organisms examined.Keywords
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