Studies with anti-bromodeoxyuridine antibodies: II. Simultaneous immunocytochemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa.
Open Access
- 1 March 1987
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 35 (3) , 371-374
- https://doi.org/10.1177/35.3.3546484
Abstract
We developed a rapid and convenient immunocytochemical method for simultaneous detection of antigen expression and S-phase cells by means of anti-bromodeoxyuridine (BrdUrd) antibodies. Immunocytochemical detection of BrdUrd after in vivo administration in mice was compared with autoradiography using [3H]-BrdUrd. Both the sensitivity and specificity of the technique were high. For the dual peroxidase staining technique, DAB color modification by cobalt ions was used. We showed that antigen localization was not affected by the BrdUrd staining protocol. The technique we describe here can be performed on frozen or paraffin-embedded tissue and on cytocentrifuge preparations for analysis of the cytokinetics of phenotypically defined cells in heterogeneous populations.This publication has 9 references indexed in Scilit:
- A monoclonal antibody reactive with 5‐bromo‐2‐deoxyuridine that does not require DNA denaturationCytometry, 1985
- Cell kinetic studies of in situ human brain tumors with bromodeoxyuridineCytometry, 1985
- Cytochemistry for bromodeoxyuridine/DNA analysis: Stoichiometry and sensitivityCytometry, 1985
- Double labeling and in vitro versus in vivo incorporation of bromodeoxyuridine in patients with acute nonlymphocytic leukemiaCytometry, 1985
- DOUBLE LABELING OF S-PHASE MURINE CELLS WITH BROMODEOXYURIDINE AND A SECOND DNA-SPECIFIC PROBE1985
- Cell Kinetic Studies In the Epidermis of Mouse. Iii. the Percent Labelled Mitosis (Plm) TechniqueCell Proliferation, 1985
- Cell‐Cycle Analysis Using A Monoclonal Antibody to BrdurdCell Proliferation, 1984
- Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.Proceedings of the National Academy of Sciences, 1983
- Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA ReplicationScience, 1982