Method for Establishing Human Cells in Culture: Susceptibility to Poliomyelitis after Prolonged Cultivation.

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Abstract
Tissue is explanted in a thin clot on roller tubes. When growth is well developed, original explants are removed. Subcultures are made by scraping cells from the walls of the tubes, and then either preparing a new culture in thin clot in a roller tube, or planting the cells directly on glass. Enough cells remain in the original roller tube to provide a new generation of cells if more nutrient fluid is added and the tubes are reincubated. The nutrient fluid consists of 85% Fisher V-614 soln., 5% beef embryo extract and 10% horse serum. Cells from the skin and muscle of a human embryo cultivated in this fashion for 6 months were still susceptible to the cyto-pathogenic effects of MEFi strain of poliomyelitis virus.

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