Direct Photolabeling with [32P]UDP-Glucose for Identification of a Subunit of Cotton Fiber Callose Synthase
Open Access
- 1 February 1991
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 95 (2) , 556-563
- https://doi.org/10.1104/pp.95.2.556
Abstract
We have identified a 52 kilodalton polypeptide as being a likely candidate for the catalytic subunit of the UDP-glucose: (1→3)-β-glucan (callose) synthase of developing fibers of Gossypium hirsutum (cotton). Such a polypeptide migrates coincident with callose synthase during glycerol gradient centrifugation in the presence of EDTA, and can be directly photolabeled with the radioactive substrate, α-[32P]UDP-glucose. Interaction with the labeled probe requires Ca2+, a specific activator of callose synthase which is known to lower the Km of higher plant callose synthases for the substrate UDP-glucose. Using this probe and several other related ones, several other proteins which interact with UDP-glucose were also identified, but none satisfied all of the above criteria for being components of the callose synthase.Keywords
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