Quantification of global transcription patterns in prokaryotes using spotted microarrays
Open Access
- 13 December 2007
- journal article
- research article
- Published by Springer Nature in Genome Biology
- Vol. 8 (12) , R265
- https://doi.org/10.1186/gb-2007-8-12-r265
Abstract
We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, that quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyze absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition.Keywords
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