Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression
- 1 December 1996
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 32 (6) , 1197-1203
- https://doi.org/10.1007/bf00041406
Abstract
Analysis of Agrobacterium-transferred DNA (T-DNA) revealed strong correlations between transgene structures and floral pigmentation patterns from chalcone synthase (chs) co-suppression among 47 Petunia transformants. Presented here are the full details of T-DNA structural organization in that population. Sixteen transformants (34%) carried one T-DNA copy while 31 (66%) carried 106 complete and partial T-DNA elements in 54 linkage groups. Thirty linkage groups contained multiple T-DNA copies; 15 of these contained only contiguously repeated copies, 8 contained only dispersed copies and 7 contained both. Right-border inverted repeats were three times more frequent than left-border inverted or direct repeats. Large fragments of binary-vector sequences were linked to the T-DNA in seven plants.Keywords
This publication has 21 references indexed in Scilit:
- Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequencesPlant Molecular Biology, 1996
- On Defining T-DNA.Plant Cell, 1994
- Stability of transgenes and presence of N6 methyladenine DNA in transformed wheat cellsThe Plant Journal, 1994
- Chronicles from the Agrobacterium-Plant Cell DNA Transfer StoryAnnual Review of Plant Biology, 1992
- Chromoplast formation during tomato fruit ripening. No evidence for plastid DNA methylationPlant Molecular Biology, 1991
- DNA variation in tissue-culture-derived rice plantsTheoretical and Applied Genetics, 1990
- Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans.Plant Cell, 1990
- Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformationPlant Molecular Biology, 1988
- Adenine methylation in zein genesBiochemical and Biophysical Research Communications, 1987
- CytologyPublished by Springer Nature ,1984