Time Normalization in Liquid Chromatography

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Abstract
Time normalization chromatography can be utilized in liquid chromatography (LC) to improve the resolution without sacrificing analysis time. This paper deals with certain aspects of time normalization in LC; namely via changes in column length, carrier velocity, solid support size, capacity ratio etc. It is shown that due to the various plate expressions which are given in the literature for the various systems in LC, different optimization criteria are best suited for each type of efficiency behavior, for each of the normalization methods. Two important methods of normalization; namely changes in the temperature or the solvent strength of the mobile phase concurently with one other parameter are discussed briefly. The practical and experimental aspects of time normalization are discussed in the Summary section.

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