Binding of Ala‐scanning analogs of ω‐conotoxin MVIIC to N‐ and P/Q‐type calcium channels
- 8 March 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 469 (2-3) , 147-150
- https://doi.org/10.1016/s0014-5793(00)01263-1
Abstract
Omega-Conotoxin MVIIC binds to P/Q-type calcium channels with high affinity and N-type channels with low affinity. To reveal the residues essential for subtype selectivity, we synthesized Ala-scanning analogs of MVIIC. Binding assays using rat cerebellar P(2) membranes suggested that Thr(11), Tyr(13) and Lys(2) are essential for binding to both N- and P/Q-type channels, whereas Lys(4) and Arg(22) are important for binding to P/Q-type channels. These results suggest that MVIIC interacts with P/Q-type channels via a large surface, in good agreement with previous observations using chimeric analogs.Keywords
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