Concerted Induction of β-Glucanases of Bacillus circulans WL 12 in Response to Various Yeast Glucans

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Abstract
Bacillus circulans WL 12 was grown on cells of six species of yeasts as inducer substrates, and the courses of progress of β-glucanase production were compared. Also, the isoelectric focusing patterns of β-1,3 and β-1,6 glucanases were determined. Conspicuous differences were observed among the isoelectric focusing patterns of the crude enzymes produced with different yeast species as inducer substrates. Lyses of the cell walls of Pyricularia oryzae P2 and several species of yeasts by the enzyme systems were compared. The lysis of the cell walls of Debaryomyces hansenii was stronger in the order of the complexity (number of major peaks etc.) of the β-1,3 glucanases in isoelectric focusing patterns.