Comparison of the API 20S Streptococcus identification system with an immunorheophoresis procedure and two commercial latex agglutination tests for identifying beta-hemolytic streptococci

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Abstract
The API 20S Streptococcus identification system and a new immunorheophoresis procedure were evaluated as means for determining the Lancefield serogroup of .beta.-hemolytic streptococci recovered from human clinical specimens. The serogroup of 96 strains was determined by these methods and by 2 commercially available latex agglutination tests, Streptex and SeroSTAT. The results of all 4 procedures were compared with the results of a classical precipitin test. The API 20S system correctly categorized 92.7% of the isolates; 94.8% were correctly identified by the immunorheophoresis procedure. The latex agglutination procedures were of comparable accuracy, yielding correct identifications with .apprx. 92% of the strains tested.