Characterization of a new family of proteins that interact with the C‐terminal region of the chromatin‐remodeling factor CHD‐31
- 3 December 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 533 (1) , 14-20
- https://doi.org/10.1016/s0014-5793(02)03737-7
Abstract
The two human proteins Ki-1/57 and CGI-55 have highly similar amino acid sequences but their functions are unknown. We analyzed them by yeast two-hybrid screens and found that they interact with the C-terminal region of the human chromatin-remodeling factor CHD-3 (chromo-helicase-DNA-binding domain protein-3). The interaction of CGI-55 and CHD-3 could be confirmed in vitro and in vivo by co-immunoprecipitations from Sf9 insect cells. Mapping showed that CGI-55 interacts with CHD-3 via two regions at its N- and C-terminals. The CGI-55 and Ki-1/57 mRNAs show highest expression in muscle, colon and kidney. A CGI55-GFP fusion protein was localized in the cytoplasm, nucleus and perinuclear regions of HeLa cells. These data suggest the possibility that CGI-55 and Ki-1/57 might be involved in nuclear functions like the remodeling of chromatin.Keywords
This publication has 26 references indexed in Scilit:
- Identification and cDNA Cloning of a Novel RNA-binding Protein That Interacts with the Cyclic Nucleotide-responsive Sequence in the Type-1 Plasminogen Activator Inhibitor mRNAJournal of Biological Chemistry, 2001
- Identification of a human 17p‐located cDNA encoding a protein of the Snf2‐like helicase familyEuropean Journal of Biochemistry, 1998
- The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.Genes & Development, 1993
- Chromatin as an essential part of the transcriptional mechanimNature, 1992
- Protein kinase activity of the intracellular but not of the membrane-associated form of the KI-1 antigen (CD30)Research in Immunology, 1990
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- The Hodgkin-Associated Ki-1 Antigen Exists in an Intracellular and a Membrane-Bound FormBiological Chemistry Hoppe-Seyler, 1989
- Modelling the ATP‐binding site of oncogene products, the epidermal growth factor receptor and related proteinsFEBS Letters, 1984
- Predicted nucleotide-binding properties of p21 protein and its cancer-associated variantNature, 1983
- Production of a monoclonal antibody specific for Hodgkin and Sternberg–Reed cells of Hodgkin's disease and a subset of normal lymphoid cellsNature, 1982