Lack of Specificity of Lymphocyte-Mediated Cytotoxicity Against the Bladder Cancer Cell Line, T24

Publisher Website
Google Scholar
Abstract
In vitro lymphocyte mediated cytotoxicity against the T24 transitional carcinoma cell line and a control melanoma cell line (H 894) was evaluated in a double-blind study involving 25 bladder cancer patients, 19 patients with nontransitional carcinoma and 9 patients with benign conditions using a 3H proline-labeled tumor cell assay. Selective reactivity was found against T24 cells in 16% of bladder cancer patients, 11% of patients with nontransitional cell tumors and 22% of patients with benign conditions. No significant differences were found with respect to the patterns of cytotoxic reactivity among the various patient groups. The use of different methods of lymphocyte purification and different lymphocyte-to-target cell ratios did not enhance the degree of specificity observed. Prior exposure to alloantigens did not account for the lack of specificity.