Changes in legumin messenger RNAs throughout seed development in Pisum sativum L.
- 1 April 1987
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 170 (4) , 562-566
- https://doi.org/10.1007/bf00402992
Abstract
The patterns of accumulation of three classes of legumin mRNA from Pisum sativum have been followed through seed development by cell-free translation and hybridization to complementary DNAs. Maximum amounts of mRNA were found at 19 days after flowering (DAF) for two classes and at 24 DAF for the third class. The proportions of the three classes varied through development: the RNA species which was 40% of the total legumin mRNA at 14 DAF represented 15–20% of the total at 25–27 DAF, whereas a second mRNA species represented approx. 30% and 70% at the same stages, respectively. Based on these results, some deductions about the possible contribution of individual genes within classes are made.Keywords
This publication has 25 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Influence of Sulfur Nutrition on Developmental Patterns of Some Major Pea Seed Proteins and Their mRNAsPlant Physiology, 1984
- Storage protein precursor polypeptides in cotyledons of Pisum sativum L.. Identification of, and isolation of a cDNA clone for, an 80000-Mr legumin-related polypeptideEuropean Journal of Biochemistry, 1984
- Inheritance and mapping of storage protein genes in Pisum sativum L.Heredity, 1982
- Molecular and Subunit Heterogeneity of Legumin ofPisum sativumL. (Garden Pea)— A Multi-Dimensional Gel Electrophoretic StudyJournal of Experimental Botany, 1981
- Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-1,5-bisphosphate carboxylaseNature, 1980
- Genetic variability in the structure of the α-subunits of legumin from Pisum—a two-dimensional gel electrophoresis studyHeredity, 1979
- Cotyledonary Storage Proteins in Pisum Sativum. II. Hereditary Variation in Components of the Legumin and Vicilin FractionsFunctional Plant Biology, 1978
- Cotyledonary Storage Proteins in Pisum sativum. I. Molecular HeterogeneityFunctional Plant Biology, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977