Spleen DPNH Cytochrome c Reductase Activity In X-Irradiated Rats.

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Abstract
Rats were sub-jected to 700 r of whole-body X-irradiation. From 13 to 85 hours after exposure, 4 subcellular fractions of X-irradiated and control spleen homogenates were made. Per mg of nitrogen, DPNH cytochrome c reductase activity of the homogenates from the X-rayed rats increased significantly over the control levels at 13 and 37 hours after exposure. At 85 hours, the difference was much less owing to an increased specific activity of the enzyme in the control homogenates. Expressed on the basis of nitrogen, the cytochrome reductase specific activities of the nuclear, microsomal, and supernatant fractions after irradiation showed no striking changes as compared to the control values. The specific activities of the mitochondrial fractions from the spleens of the irradiated animals decreased significantly as compared to the control levels but it is likely that this was due, in part, to the fact that the percentage of whole homogenate N recovered in the mitochondria after irradiation was twice that found in the mitochondria derived from the spleens of control rats. The percentage of total DPNH cytochrome c reductase activity recovered in the 4 fractions prepared from the spleens of irradiated and control animals was essentially the same.

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