Molecular cloning of two cDNAs from the head of the nurse honey bee (Apis melliferaL.) for coding related proteins of royal jelly

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Abstract
Two cDNA clones designated pRJP57–1 and pRJP57–2 which code for related polypeptides of major royal jelly protein were identified from the λUni-ZAP XR library of nurse honey bee head cDNA. The nucleotide sequences of the cDNA inserts show a 70.2% identity. From the open reading frames of pRJP57–1 and pRJP57–2 the amino acid sequences for polypeptides of 467 and 464 amino acid residues were derived, showing an identity of 56.6%. No significant homology with hitherto known proteins was observed. Both proteins contain N-glycosylation sites, phosphorylation sites for three types of protein kinases and almost identical signal peptides. Northern analysis showed that total mRNA contains a high proportion of RJP57–1 and RJP57–2 mRNAs (8% and 2% respectively).