Polyamine metabolism is upregulated in response to tobacco mosaic virus in hypersensitive, but not in susceptible, tobacco
- 1 February 2001
- journal article
- research article
- Published by Wiley in New Phytologist
- Vol. 149 (2) , 301-309
- https://doi.org/10.1046/j.1469-8137.2001.00017.x
Abstract
• Change is reported in the biosynthetic and oxidative activity of hypersensitive (NN) and susceptible (nn) tobacco (Nicotiana tabacum) plants in response to tobacco mosaic virus (TMV). • Mature leaves of nn and NN tobacco were collected over 0-72 h as uninoculated controls or after inoculation with TMV or phosphate buffer (mock-inoculation). The polyamine response to inoculation was analysed by measuring activity and gene expression of S-adenosylmethionine decarboxylase (SAMDC), arginine-(ADC) and ornithine decarboxylases (ODC); incorporation of labelled putrescine; and activity of diamine oxidase (DAO). • In NN leaves SAMDC activity and transcript levels, and DAO activity increased in the TMV-inoculated plants but not in the other treatments; a two-fold increase in DAO activity was seen after 72 h. Both ADC and ODC activity increased in NN leaves at 72 h in TMV-inoculated plants; ADC mRNA increased with activity. The increase in SAMDC mRNA (24 h) preceded the rise in activity (72 h). [3 H]putrescine added to NN leaves led to enhanced label recovery and incorporation into spermidine and spermine in TMV-inoculated plants. No significant changes in biosynthetic or oxidative activity occurred in nn plants. • After TMV inoculation, NN, unlike nn, tobacco plants upgrade polyamine synthesis and oxidation; this leads to changes in cellular components which might induce programmed cell death.Keywords
This publication has 40 references indexed in Scilit:
- Organogenesis fromSolanum melongena L. (eggplant) cotyledon expiants is associated with hormone-modulated enhancement of polyamine biosynthesis and conjugationProtoplasma, 2000
- Methylglyoxal(bis-guanylhydrazone) inhibition of organogenesis is not due to S-adenosylmethionine decarboxylase inhibition/polyamine depletion in tobacco thin layersPhysiologia Plantarum, 1999
- Short‐term polyamine response in TMV‐inoculated hypersensitive and susceptible tobacco plantsNew Phytologist, 1998
- Characterization of Spermidine Binding to Solubilized Plasma Membrane Proteins from Zucchini Hypocotyls1Plant Physiology, 1998
- Control of plant disease by perturbation of fungal polyamine metabolismPhysiologia Plantarum, 1997
- Polyamine synthesis and accumulation in the hypersensitive response to TMV in Nicotiana tabacumNew Phytologist, 1997
- Diamine oxidation and alkaloid production in transformed root cultures of Nicotiana tabacumPhytochemistry, 1990
- Effects of the Suicide Inhibitors of Arginine and Ornithine Decarboxylase Activities on Organogenesis, Growth, Free Polyamine and Hydroxycinnamoyl Putrescine Levels in Leaf Explants of Nicotiana Xanthi n.c. Cultivated in Vitro in a Medium Producing Callus FormationPlant Physiology, 1989
- The Synthesis of Polyamines from Methionine in Intact and Disrupted Leaf Protoplasts of Virus-Infected Chinese CabbagePlant Physiology, 1981
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976