Introduction of foreign genes into potato cultivars Bintje and D sir e using an Agrobacterium tumefaciens binary vector
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 7 (1) , 47-50
- https://doi.org/10.1007/bf00272976
Abstract
Tuber discs of Solanum tuberosum cv Bintje and Désirée were cocultivated with an Agrobacterium tumefaciens binary vector, carrying both the neomycine phosphotransferase and the E. coli β-glucuronidase gene fused to resp. the nopaline synthase and Cauliflower mosaic virus 35S promotor. Inoculated tuber discs produce transgenic shoots in selective media containing kanamycin. The transgenic plants are phenotypically normal and contain the euploid number of chromosomes. Both the neomycin phosphotransferase as well as the β-glucuronidase gene are expressed conferring resp. kanamycin resistance and β-glucuronidase activity to the plants.This publication has 41 references indexed in Scilit:
- Engineering Herbicide Tolerance in Transgenic PlantsScience, 1986
- Photosynthesis-Associated Gene Families: Differences in Response to Tissue-Specific and Environmental FactorsScience, 1986
- Delay of Disease Development in Transgenic Plants That Express the Tobacco Mosaic Virus Coat Protein GeneScience, 1986
- Transformation of Tobacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector SystemPlant Physiology, 1986
- Expression of biologically active viral satellite RNA from the nuclear genome of transformed plantsNature, 1986
- A Simple and General Method for Transferring Genes into PlantsScience, 1985
- A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmidNature, 1983
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cytokinins and Tuber Initiation in the Potato Solanum tuberosum L.Nature, 1969