Synergistic Action of Angiotensin II, Insulin- Like Growth Factor-I, and Transforming Growth Factor-β on Platelet-Derived Growth Factor-BB, Basic Fibroblastic Growth Factor, and Epidermal Growth Factor-Induced DNA Synthesis in Vascular Smooth Muscle Cells
The effect of angiotensin II (AII), transforming growth factor-β (TGF-β), and insulin-like growth factor-I (IGF-I) on platelet-derived growth factor(PDGF)-BB-, basic fibroblastic growth factor(bFGF)-, and epidermal growth factor (EGF)-inducedDNA synthesis in vascular smooth musclecells (VSMCs) was investigated. TGF-β AII, IGF-Iincreased concentration-dependent cell protein. No increase in [3H]thymidine incorporation could be detected. TGF-β, AII, and IGF-I enhanced PDGFBB-, bFGF-, and EGF-induced DNA synthesis. Our results suggest that growth factors that possess a weak mitogenic effect on vascular smooth muscle cells such as TGF-β, AII, and IGF-I may enhance the mitogenicity of PDGF-BB, bFGF, and EGF. AmJ Hypertens 1993;6:496-499