THE STUDY OF SERUM PROTEINS AND LIPIDS WITH THE AID OF THE QUANTITY ULTRACENTRIFUGE. I. PROCEDURE AND PRINCIPAL FEATURES OF THE CENTRIFUGATE OF UNTREATED NORMAL SERUM AS DETERMINED BY QUANTITATIVE ANALYSIS OF SAMPLES FROM TEN LEVELS 12

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Abstract
A procedure for the study of untreated human sera with the quantity ultra-centrifuge is described, in which the column of ultracentrifugate is sampled at 10 levels and analyzed by quantitative methods for proteins and lipids. Detailed results of analyses of centrifugate and whole sera of 14 normal men are shown in tables which also indicate overall variability of concn. Graphs present mean curves of distribution in the column of centrifugate of protein and lipid constituents after regular and prolonged centrifugation. Zone of minimum density at the top of the column contains about 1/2 of whole serum neutral fat which is the principal constituent. Zone of low-medium density in mid-column contains nearly 1/2 the lipids in the serum, principally responsible for the variation in concns. of most lipids of normal serum. The distribution of lipids in the lower portion of the centrifugate shows the presence there of serum lipoproteins containing either phospholipids or neutral fat or perhaps both; in one type the cholesterol is only of the esterified form and in the other there is no cholesterol whatever.