The kinetics of fluoride binding by lactoperoxidase
- 1 December 1968
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 46 (12) , 1471-1474
- https://doi.org/10.1139/o68-219
Abstract
The kinetics of the reversible binding of fluoride by ferric lactoperoxidase has been studied over the pH range 3.8–5.4 by means of a stopped-flow apparatus. Because of small absorbance changes upon the binding of fluoride by lactoperoxidase, the reaction was driven to completion by addition of a large excess of fluoride. Under these conditions the binding reaction obeys pseudo-first-order kinetics and an accurate analysis of the dissociation rate data is not possible. The results are consistent with a mechanism in which HF binds to the active site which contains no groups capable of ionization in the pH range of the present study. The specific rate constant for the binding reaction of HF to lactoperoxidase is (9.7 ± 0.4) × 102 M−1s−1.Keywords
This publication has 0 references indexed in Scilit: