Spray Methods for the Detection of 2-Amino-2-deoxyhexoses and their N-Acetyl Derivatives on Paper Chromatograms

Publisher Website
Google Scholar
Abstract
The simultaneous detection of 2-amino-2-deoxy-hexose and their N-acetyl derivatives on paper chromatograms has been made more sensitive and specific by the use of a potassium tetraborate-ethanol spray and an acetic anhydride-acetone spray, followed by heating and spraying with a p-dimethylaminobenzaldehyde spray. As little as 0.7γ of glucosamine hydrochloride and 0.8γ of N-acetylglucosamine can be detected on papers. The specific detection of 2-acetamino-2-deoxy-hexoses on paper chromatograms has been made more sensitive by the use of the potassium tetraborate-ethanol spray, followed by heating and spraying with the p-dimethylaminobenzaldehyde spray. As little as 1.1γ of N-acetylglucosamine can be detected on papers.