A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues
- 1 July 1997
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 115 (1) , 29-32
- https://doi.org/10.1017/s0031182097001091
Abstract
A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.Keywords
This publication has 0 references indexed in Scilit: