Quantal release of acetylcholine examined by current fluctuation analysis at an identified neuro-neuronal synapse of Aplysia.
- 1 March 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (3) , 1661-1665
- https://doi.org/10.1073/pnas.77.3.1661
Abstract
Quantal events were analyzed at a neuro-neuronal synapse of A. californica where the nature of the transmitter is established and both presynaptic and postsynaptic neurons are identified and can be voltage-clamped. Prolonged depolarizations were applied to the presynaptic neuron, which gave rise in the postsynaptic cell to a current response characterized by current fluctuations or noise. Acetylcholine was also applied ionophoretically on the same postsynaptic cell. Amplitude and time course of miniature currents and acetylcholine-activated chloride channels of the same cell were examined by using a current fluctuation analysis. One presynaptic spike releases about 180 quanta, each opening 500 chloride channels.Keywords
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