A Non-Vacuum Freezing-Dehydrating Technic for Histology, Autoradiography and Microbial Cytology
- 1 January 1951
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 26 (3) , 193-197
- https://doi.org/10.3109/10520295109113207
Abstract
A method for the freezing-dehydration of tissue and microbial cells is described which circumvents the need for high vacuum for the purpose of removing water from the cells by direct vaporization of ice. The present method utilizes the dissolution of ice from frozen tissue and cells by immersion in chemical dehydrating agents which remain liquid at low temperatures. Choice of the particular agent depends upon the desired result. Glycols are recommended if fixation is to be avoided, or water-soluble isotopes retained in the cells; alcohols or acetone are used if fixation is not undesirable. Specific applications of the method are given for use with tissues and with microbial cells. It has been demonstrated that radioactive phosphorus (P32) is not leached out of the tissues during the procedure.Keywords
This publication has 6 references indexed in Scilit:
- ELECTRON AND LIGHT MICROSCOPIC STUDIES OF BACTERIAL NUCLEI IIJournal of Bacteriology, 1950
- The Chemistry of the Feulgen Reaction and Related Histo- and Cytochemical Methods*Journal of Investigative Dermatology, 1950
- Autoradiography of Water-Soluble Tracers in Histological SectionsNature, 1950
- Preparation of Radioautographs of Tissues without Loss of Water-Soluble P 32Science, 1949
- Preparation of Radioautographs to Show the Distribution of Phosphorus-32 in Plant TissuesNature, 1949
- A Rapid Imbedding Technic for Histologic Sections Employing a Water Soluble Wax11This study was supported by a grant-in-aid from the United States Public Health Service.Journal of Investigative Dermatology, 1949