Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.
Open Access
- 1 February 1989
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 9 (2) , 415-420
- https://doi.org/10.1128/mcb.9.2.415
Abstract
A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction of expression of RTA was lethal. This lethality was the basis for a selection of mutations in RTA which inactivated the toxin. A number of mutant alleles which encoded cross-reactive material were sequenced. Eight of the first nine mutant RTAs studied showed single-amino-acid changes involving residues located in the proposed active-site cleft.This publication has 22 references indexed in Scilit:
- A rapid boiling method for the preparation of bacterial plasmidsPublished by Elsevier ,2004
- Expression of ricin A chain in Escherichia coliFEBS Letters, 1987
- Use of lacZ fusions to delimit regulatory elements of the inducible divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1984
- Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1984
- RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.Proceedings of the National Academy of Sciences, 1983
- Construction, replication, and chromatin structure of TRP1 RI circle, a multiple-copy synthetic plasmid derived from Saccharomyces cerevisiae chromosomal DNA.Molecular and Cellular Biology, 1982
- Entry of lethal doses of abrin, ricin and modeccin into the cytosol of HeLa cellsExperimental Cell Research, 1980
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Treatment of abrin and ricin with β‐mercaptoethanol. Opposite effects on their toxicity in mice and their ability to inhibit protein synthesis in a cell‐free systemFEBS Letters, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970