The CCK receptor on pancreatic plasma membranes: binding characteristics and covalent cross‐linking
- 10 January 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 151 (1) , 63-66
- https://doi.org/10.1016/0014-5793(83)80343-3
Abstract
The cholecystokinin (CCK) receptor in purified plasma membranes prepared from mouse pancreatic acini had a binding affinity of 1.8 nM, an acid pH optimum between 6.0 and 6.5, and an analog specificity of CCK8 > CCK33 > desulphated CCK8 > CCK4. Binding of CCK to its receptor was abolished by pretreatment of plasma membranes with trypsin. When [125I]CCK was cross-linked to its receptors with disuccinimidyl suberate, and the preparation solubilized and subjected to gel electrophoresis and autoradiography, the hormone was associated with Mr 80000 protein in both the presence and absence of the reducing agent dithiothretolKeywords
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