Analysis of Plasmalogens byInSituReaction on Thin Layer Chromatograms

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Abstract
An “in situ” hydrolysis method that attacks only the enol ether double bond of plasmalogen is described. Alkyl and unsaturated phosphollpids are not affected. Treatment of the plasmalogen on thin layers of silica gel with solutions of trichloroacetic acid in dilute hydrochloric acid result in more complete hydrolysis of the vinyl-ether linkage. After development of the chromatogram differential quantitation using copper sulfate charring (1) and densitometry will give the amount of vinyl-ether lipid present in the sample. The results of the method with ethanolamine plasmalogen are presented.