An Artifact in the ESR Spectrum Obtained by Spin Trapping with Dmpo
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 6 (1) , 19-28
- https://doi.org/10.3109/10715768909073424
Abstract
In order to overcome a common problem in spin trapping with high concentrations of 5.5-dimethyl-I-pyrroline-N-oxide (DMPO) where ESR spectra are obtained which include an unidentified set of lines composed of a triplet of doublets. commercial DMPO was analyzed for its impurities by high-performance liquid chromatography. mass spectrometry. and nuclear magnetic resonance spectroscopy. It has been determined that this undesirable ESR spectrum IS due to an impurity included in the spin trap. This compound has been assigned to the hydronylunine which is a DMPO-derivative having an epoxy ring located at the 2 and 3 positions.Keywords
This publication has 10 references indexed in Scilit:
- Spin trapping in biological systems. Oxidation of the spin trap 5,5-dimethyl-1-pyrroline-1-oxide by a hydroperoxide-hematin systemPublished by Elsevier ,2004
- An ESR study on lipid peroxidation process. Formation of hydrogen atoms and hydroxyl radicalsBiochemical and Biophysical Research Communications, 1986
- Formation of ∙ OH and ∙ H in Aqueous Solutions by Ultrasound Using Clinical EquipmentRadiation Research, 1983
- Oxygen-derived radicals fromTrypanosoma cruzi-stimulated human neutrophilsFEBS Letters, 1983
- Chemical effects of ultrasound on aqueous solutions. Formation of hydroxyl radicals and hydrogen atomsThe Journal of Physical Chemistry, 1983
- Chemical effects of ultrasound on aqueous solutions. Evidence for hydroxyl and hydrogen free radicals (.cntdot.OH and .cntdot.H) by spin trappingJournal of the American Chemical Society, 1982
- Spin trapping. Kinetics of the reaction of superoxide and hydroxyl radicals with nitronesJournal of the American Chemical Society, 1980
- Spin trapping of superoxide and hydroxyl radical: Practical aspectsArchives of Biochemistry and Biophysics, 1980
- Effects of ultrasound on nucleic acid basesBiochemistry, 1977
- 424. Experiments towards the synthesis of corrins. Part III. Formation of a bicyclic oxaziran from a Δ1-pyroline and from the corresponding nitroneJournal of the Chemical Society, 1959