THE HISTOCHEMISTRY OF CONNECTIVE TISSUE: II. THE EFFECT OF PROTEINS ON THE SELECTIVE STAINING OF MUCOPOLYSACCHARIDES BY BASIC DYES
Open Access
- 1 September 1953
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 1 (5) , 321-325
- https://doi.org/10.1177/1.5.321
Abstract
The expts. performed demonstrated that proteins may compete with mucopolysaccharides for basic dyes. This competition leads to the following implications when basic dyes are employed as histochemical reagents: (1) acidic character of a mucopolysaccharide may be partly or completely masked by the presence of basic proteins, (2) the pH dependence of the staining of mucopolysaccharides may more truly reflect this competition rather than the dissociation groups of the polysaccharide, and (3) proteins in tissue extracts may simulate hyaluronidase or chondroitinase activity by blocking stainable groups.Keywords
This publication has 1 reference indexed in Scilit:
- TOLUIDINE BLUE BINDING BY DEVELOPING MUSCLE TISSUE: ASSAY AND DATA ON THE MECHANISM INVOLVEDJournal of Biological Chemistry, 1950