Abstract
A milking animal from the dairy herd of the U. Georgia was observed to become refractory to the stimuli which cause milk ejection under standard milking procedures. She was used to estimate the least amount of exogenous oxytocin which would cause normal milk ejection. This amount was 0.06 I.U. Oxytocin injected rapidly into the vein was even more effective than slowly injected oxytocin. Oxytocin treatment at either of the last 2 previous milkings affected the ejection response and account should be taken of previous treatments in evaluating responses to exogenous oxytocin. Three animals were used to determine whether relaxin ejects milk as does oxytocin. This was not so.