A Simple and Rapid Method for the Isolation of Cholesterol from Spinal Cord of Cattle
- 31 December 1976
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 7 (3-4) , 181-186
- https://doi.org/10.1080/00327487708061636
Abstract
A method is described for the large scale isolation of cholesterol from cattle spinal cord. Cholesterol is released from the lipo-protein by drying the wet tissues with calcium oxide. This treatment appears to prevent the extraction of polar lipids with acetone, which reduces the lipophilic properties of the solution and thus provides a method for almost exclusive extraction of cholesterol. The further role of calcium oxide is also discussed. The method evolved is simple, rapid and eliminates several previously required steps.This publication has 3 references indexed in Scilit:
- Synthesis of Unsaturated α-Lecithins.1 I. L-α-(Dioleyl)-lecithinJournal of the American Chemical Society, 1956
- The lipids of fish. 2. The acetone-soluble lipids of the flesh of the haddockBiochemical Journal, 1953
- Eine schnelle Methode zur Cholesterindarstellung aus Hirn.Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1936